More than 1,500 people have signed a petition calling for an end to hunting in woods around Flaxley in the Forest of Dean.
Campaigners claim members of the Cotswold Vale Farmers Hunt are illegally chasing foxes through the woods and trying to dig out badger setts.
The hunt’s riders have been filmed riding in the woods and protesters claim this has put members of the public and their pets at risk.
One of the protesters, Barbara Slaska, claimed her husband had been galloped into by a member of the hunt.
She and other residents staged a protest in the woods and started the online petition.
It calls for the Forestry Commission to stop licensing the hunt so it can no longer operate in Flaxley, Hope and Muggleworth woods.
Mrs Slaska said: “I have sent the commission evidence that the public are being put at risk since the wood is very small (circumnavigated in an hour) and cannot safely accommodate walkers, cyclists, recreational riders and the hunt along a single arterial track.”
She added: “The chasing of foxes, particularly into active badger setts, has been witnessed by locals and letters have been sent to the commission with no reply.
“Locals have also witnessed deer and hares being chased by hounds.”
Gloucestershire Live has approached the hunt for comment.
A spokeswoman for Gloucestershire police said: “On Saturday February 16 a man reported that he believed he had been assaulted by a horse or rider which passed him on a bridleway in Flaxley Woods, Newnham.
“No injuries were reported and no words were exchanged between the man and the rider.
“There were no witnesses to the incident, nor any evidence to show what happened. So officers have no viable lines of inquiry and have been unable to progress the investigation any further.
“The man who reported the incident has been informed.”
A spokeswoman for the Forestry Commission said: ““The Cotswold Vale Farmers Hunt do have a current trail hunting licence from us for Flaxley Woods and it is reviewed annually.
“If anyone has evidence that would support claims that a hunt have breached their licence or broken then law then we would urge members of the public to give us this information and report any suspected breaches of the law to the police.
“All reports or complaints we receive are held in the strictest confidence and no names would ever be disclosed to another person or organisation. Forestry Commission staff have training on the Data Protection Regulations are fully aware of their responsibilities and take them very seriously.”
She added: “We investigate complaints supported by credible evidence and facts and take action when necessary. We cannot take any action without strong evidence.
“Flaxley Wood, along with the wider Forest of Dean, is enjoyed by many different user groups. Conflicts between user groups can occur, however the woods are to be enjoyed by all and we encourage groups to respect each other’s right to enjoy the forest and work alongside each other to ensure everyone enjoys their visit.
“If a member of the public witnesses a wildlife crime, they should report it to the police in the first instance by calling 101.”